Investigation+3+-+Blast

THIS IS AN INDIVIDUAL LAB! Make a document and share with me. DO all the prelab (p43-44) on cladograms from the lab book. You can hand draw the Table 1 and Table 2 cladograms on paper and take a picture or hand them in separately. ANswer the question (a). Use these links to navigate the Blast lab. See your lab book for the details and see below for some modfications. For p.S45 :
 * Step 1-** Instead of drawing, just **describe** where you would place the fossil in the above cladogram.

**Step 2** -Download and save these [|4 gene files]to your **downloads** files. NOTE: these will not save to your desktop nor will they open until you paste them to the program. A screen will appear with the parameters for your query already configured. NOTE: Do not alter any of the parameters. Scroll down the page and click on the "BLAST" button at the bottom. After collecting and analyzing all of the data for that particular gene, repeat this procedure for the other two gene sequences.
 * HOW TO: **You must click on the files, and download them by clicking, 'Download' and then save. Once the zip file is saved, you should then unpack it by saving the individual file. Then you must go to the BLAST site (see below)and
 * 1) Click on the 'Saved Strategies' from the menu at the top of the page
 * 2) Under "Upload Search Strategy", click on "Browse" and locate one of the gene files you saved onto your computer
 * 3) Click "View"

**Step 3:** BLAST homepage. ( **//http://blast.ncbi.nlm.nih.gov/Blast.cgi//** ) Save a picture of your results and insert into your document. Follow the lab book direction p 45-48, **Answer the Analysis questions 1-4 for EACH gene** (Label them gene 1,2,3,4) Evaluating results: Share your results with one other person and discuss the questions in that section.

For p. S49 1. Entrez Gene website ( **//http://www.ncbi.nlm.nih.gov/gene//** ) NOTE: the instruction step 2 in lab book is in error- go to the right hand list and choose __reference sequence (there is__ no NCBI title).
 * Designing your Investigation: You will be assigned a gene to research**

6. BLAST homepage ( **//http://blast.ncbi.nlm.nih.gov/Blast.cgi//** ).

**Answer the 5 bulleted questions about your gene in your shared document**. Include a saved result picture as a reference.